Hyphen Biomed USP-EP Anti‐Factor Xa and Anti‐Factor IIa Assay Buffers available for Unfractionated and Low Molecular Weight Heparins

For the Anti-Xa 2-stage assay, for performing the method in compliance with the new USP or EP guidances, the needs are summarized in the table below, for LMWH or UFH:

Anti-Xa 2-stage (# 221005)Volume of buffer (for 1 mL of reagent)Buffer used
Heparin measuredLMWHUFHLMWHUFH
Sample# AR005L (EP) # AR028K (USP)# AR030K (USP and EP)
R14mL4mL# AR005L (USP and EP)# AR030K (USP and EP)
R24mL4mL# AR005L (USP and EP)# AR030K (USP and EP)
R34mL# AR029K (USP and EP)

For the Anti-IIa 2-stage assay, for performing the assay in compliance with the new USP or EP guidances, the needs are summarized in the table below:

Anti-IIa 2-stage (# 220005)Volume of buffer (for 1 mL of reagent)Buffer used
Heparin measuredLMWHUFHLMWHUFH
Sample# AR005L (EP) # AR028K (USP# AR030K (USP and EP) or # AR031K (USP)
R14mL4mL# AR005L (USP and EP)# AR030K (USP and EP) or # AR031K (USP)
R24mL4mL# AR005L (USP and EP)# AR030K (USP and EP) or # AR031K (USP)
R34mL# AR029K (USP and EP)

Smart Buffers by Medicago available in pre‐measured pouches for use with compendial tests.

For large volume industrial Heparin testing, in compliance with USP/EP guidances of anti-Xa and anti-IIa assay buffers:

Aniara SKUA12‐0018A12‐0102A12‐0103A12‐0104A12‐0105
Product NameUSP‐EP Anti‐FXa & Anti‐ FIIa Assay for UFH Buffer with PEG, pH 8.4USP Anti‐FXa & Anti‐FIIa Assay for LMWH Buffer with PEG, pH 7.4USP Anti‐FXa & Anti‐FIIa Assay for LMWH Buffer, pH 7.4USP‐EP Anti‐FXa & Anti‐ FIIa Assay for LMWH Buffer, pH 8.4USP‐EP Anti‐FXa & Anti‐ FIIa Assay for LMWH Buffer with BSA, pH 7.4
NaCl0.175 M0.150 M0.150 M0.175 M0.150 M
Tris0.050 M0.050 M0.050 M0.05 M0.050 M
EDTA0.0075 M

‐

‐

0.0075 M

‐

Polyethylene glycol 60000.10%0.10%

‐

‐

‐

Bovine serum albumin

‐

‐

‐

‐

1.0%
pH8.47.47.48.47.4
Final volume1 L1 L1 L0.5 L1 L

*PEG or BSA buffer can be used in USP test for LMWH.

Use charts below to determine the buffer needed for specific assay.

Product NameUSP‐EP Anti‐FXa & Anti‐ FIIa Assay for UFH Buffer with PEG, pH 8.4USP Anti‐FXa & Anti‐FIIa Assay for LMWH Buffer with PEG, pH 7.4USP Anti‐FXa & Anti‐FIIa Assay for LMWH Buffer, pH 7.4USP‐EP Anti‐FXa & Anti‐ FIIa Assay for LMWH Buffer, pH 8.4USP‐EP Anti‐FXa & Anti‐ FIIa Assay for LMWH Buffer with BSA, pH 7.4
USP Xa‐UFH
USP IIa‐UFH
USP Xa‐LMWH
USP IIa‐LMWH
USP Use:Dilution Buffer for Standards, Samples, AT, FXa, Thrombin, BlankDilution Buffer* for AT, FXa, ThrombinDilution Buffer for Standards, Samples, BlankDilution Buffer for SubstrateDilution Buffer* for AT, FXa, Thrombin
Product NameUSP‐EP Anti‐FXa & Anti‐ FIIa Assay for UFH Buffer with PEG, pH 8.4USP Anti‐FXa & Anti‐FIIa Assay for LMWH Buffer with PEG, pH 7.4USP Anti‐FXa & Anti‐FIIa Assay for LMWH Buffer, pH 7.4USP‐EP Anti‐FXa & Anti‐ FIIa Assay for LMWH Buffer, pH 8.4USP‐EP Anti‐FXa & Anti‐ FIIa Assay for LMWH Buffer with BSA, pH 7.4
EP Xa‐UFH
EP II‐UFH
EP Xa‐LMWH
EP IIa‐LMWH
EP Use:Dilution Buffer for Standards, Samples, AT, FXa, Thrombin, BlankDilution Buffer for SubstrateDilution Buffer for Standards, Samples, AT, FXa, Thrombin, Blank